Background: Tuberculosis (TB) is one of the major public health problems in Bangladesh. Still, its remains a big challenges in different area, especially in TB diagnosis.

Aims and Objectives: The aim of this study was to determine the good knowledge regarding TB which was measured by a composite index and its association with different socio-economic and demographic factors among industrial labors in Rajshahi district, Bangladesh.

Materials and Methods: A cross-sectional study was performed among 1,225 industrial labors from 75 different industries in Rajshahi district, Bangladesh. Knowledge regarding TB was the outcome of interest in this study, which was assessed through 11 different questions.

Results: This study revealed that 30.4% respondents had a poor knowledge regarding TB. Logistic regression model demonstrated that good knowledge regarding TB was especially found among labors who were doing the job in silk industries, young adults, and female, educated, non-smokers, living in poor family and living without their partners (separated). Labors who had been suffering from the non-communicable disease were more likely to have a good TB knowledge compared with their counterpart.

Conclusion: This study found that there is a huge gap in TB knowledge among industrial labors in Rajshahi district. It was noted that some modifiable factors were associated with TB knowledge. These factors can be considered to increase TB knowledge among industrial labors in Bangladesh.